Plasmid DNA (pDNA) expression vectors are fundamental to all forms of non-viral gene transfer. In this review, we discuss principles of pDNA design and production including the impact of bacterially derived sequences on transgene expression and minicircle approaches to minimize their effects. The impact of inclusion of DNA elements such as scaffold matrix attachment regions (S/MARs), transcription factor (TF)-binding sites and tissue-specific promoters are described. The benefits of eliminati...Expand abstract
- Publication status:
- Publisher copy:
- Copyright date:
Progress and prospects: the design and production of plasmid vectors.
Views and Downloads
If you are the owner of this record, you can report an update to it here: Report update to this record