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Journal article

Progress and prospects: the design and production of plasmid vectors.

Abstract:

Plasmid DNA (pDNA) expression vectors are fundamental to all forms of non-viral gene transfer. In this review, we discuss principles of pDNA design and production including the impact of bacterially derived sequences on transgene expression and minicircle approaches to minimize their effects. The impact of inclusion of DNA elements such as scaffold matrix attachment regions (S/MARs), transcription factor (TF)-binding sites and tissue-specific promoters are described. The benefits of eliminati...

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Publication status:
Published

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Publisher copy:
10.1038/gt.2008.183

Authors


Journal:
Gene therapy
Volume:
16
Issue:
2
Pages:
165-171
Publication date:
2009-02-05
DOI:
EISSN:
1476-5462
ISSN:
0969-7128
URN:
uuid:734998e3-6657-46a8-bedb-44001caf0ece
Source identifiers:
246580
Local pid:
pubs:246580

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