- Abstract:
-
Plasmid DNA (pDNA) expression vectors are fundamental to all forms of non-viral gene transfer. In this review, we discuss principles of pDNA design and production including the impact of bacterially derived sequences on transgene expression and minicircle approaches to minimize their effects. The impact of inclusion of DNA elements such as scaffold matrix attachment regions (S/MARs), transcription factor (TF)-binding sites and tissue-specific promoters are described. The benefits of eliminati...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1038/gt.2008.183
-
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- Journal:
- Gene therapy
- Volume:
- 16
- Issue:
- 2
- Pages:
- 165-171
- Publication date:
- 2009-02-05
- DOI:
- EISSN:
-
1476-5462
- ISSN:
-
0969-7128
- URN:
-
uuid:734998e3-6657-46a8-bedb-44001caf0ece
- Source identifiers:
-
246580
- Local pid:
- pubs:246580
- Copyright date:
- 2009
Journal article
Progress and prospects: the design and production of plasmid vectors.
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