Journal article
Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro.
- Abstract:
- Fluorescence recovery after photobleaching (FRAP) is a microscopy-based technique to study the movement of fluorescent molecules inside a cell. Although initially developed to investigate intracellular mobility, FRAP can be also used to measure intercellular dynamics. This chapter describes how to perform FRAP experiment to study gap junctional communication in living cells. The procedures described here can be carried out with a laser-scanning confocal microscope and any in vitro cultured cells known to communicate via gap junctions. In addition, the method can be easily adjusted to measure gap junction function in 3D cell cultures as well as ex vivo tissue.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Accepted manuscript, pdf, 525.8KB, Terms of use)
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- Publisher copy:
- 10.1007/978-1-4939-3664-9_12
Authors
- Publisher:
- Springer
- Journal:
- Methods in Molecular Biology More from this journal
- Volume:
- 1437
- Pages:
- 171-179
- Publication date:
- 2016-05-21
- Acceptance date:
- 2016-03-29
- DOI:
- EISSN:
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1940-6029
- ISSN:
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1064-3745
- Language:
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English
- Keywords:
- Pubs id:
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pubs:625033
- UUID:
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uuid:71d40cfe-c054-47e0-8851-d1879cd9ca0c
- Local pid:
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pubs:625033
- Source identifiers:
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625033
- Deposit date:
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2016-07-19
- ARK identifier:
Terms of use
- Copyright holder:
- Springer
- Copyright date:
- 2016
- Notes:
- © Springer Science+Business Media New York 2016. This is the accepted manuscript version of the article. The final version is available online from Springer at: [10.1007/978-1-4939-3664-9_12]
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