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Complete enzyme set for chlorophyll biosynthesis in Escherichia coli

Abstract:

Chlorophylls are essential cofactors for photosynthesis, which sustains global food chains and oxygen production. Billions of tons of chlorophylls are synthesized annually, yet full understanding of chlorophyll biosynthesis has been hindered by the lack of characterization of the Mg–protoporphyrin IX monomethyl ester oxidative cyclase step, which confers the distinctive green color of these pigments. We demonstrate cyclase activity using heterologously expressed enzyme. Next, we assemble a ge...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1126/sciadv.aaq1407

Authors


Canniffe, DP More by this author
Barnett, SFH More by this author
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
Brindley, AA More by this author
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Publisher:
American Association for the Advancement of Science Publisher's website
Journal:
Science Advances Journal website
Volume:
4
Issue:
1
Pages:
Article: eaaq1407
Publication date:
2018-01-26
Acceptance date:
2017-12-28
DOI:
ISSN:
2375-2548
Pubs id:
pubs:827634
URN:
uri:7176a85c-53a0-4b42-9c55-a3a4086893eb
UUID:
uuid:7176a85c-53a0-4b42-9c55-a3a4086893eb
Local pid:
pubs:827634

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