Preparation and preclinical evaluation of humanised A33 immunoconjugates for radioimmunotherapy.

Journal article

A humanised IgG1/k version of A33 (hA33) has been constructed and expressed with yields up to 700 mg l-1 in mouse myeloma NS0 cells in suspension culture. The equilibrium dissociation constant of hA33 (KD = 1.3 nM) was shown to be equivalent to that of the murine antibody in a cell-binding assay. hA33 labelled with yttrium-90 using the macrocyclic chelator 12N4 (DOTA) was shown to localise very effectively to human colon tumour xenografts in nude mice, with tumour levels increasing as blood c...

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Authors: King, D; Antoniw, P; Owens, R; Adair, JR; Haines, A

• Publication status: Published
• Journal: British journal of cancer
• Volume: 72
• Issue: 6
• Pages: 1364-1372
• Publication date: 1995-12-01
• DOI: 10.1038/bjc.1995.516
• EISSN: 1532-1827
• ISSN: 0007-0920
• Language: English
• Keywords: Tissue Distribution; Genes, Immunoglobulin; Hybridomas; Cricetinae; Yttrium Radioisotopes; Gene Expression; Mice; Multiple Myeloma; DNA, Complementary; Immunoglobulin G; Molecular Sequence Data; Neoplasm Transplantation; Drug Screening Assays, Antitumor; Immunoconjugates; Indium Radioisotopes; Mice, Nude; Immunoglobulin Fragments; Cloning, Molecular; CHO Cells; Colorectal Neoplasms; Humans; Amino Acid Sequence; Animals; Transplantation, Heterologous; Tumor Cells, Cultured