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Journal article

Preparation and preclinical evaluation of humanised A33 immunoconjugates for radioimmunotherapy.

Abstract:

A humanised IgG1/k version of A33 (hA33) has been constructed and expressed with yields up to 700 mg l-1 in mouse myeloma NS0 cells in suspension culture. The equilibrium dissociation constant of hA33 (KD = 1.3 nM) was shown to be equivalent to that of the murine antibody in a cell-binding assay. hA33 labelled with yttrium-90 using the macrocyclic chelator 12N4 (DOTA) was shown to localise very effectively to human colon tumour xenografts in nude mice, with tumour levels increasing as blood c...

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Publication status:
Published

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Publisher copy:
10.1038/bjc.1995.516

Authors


Antoniw, P More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Biology
Haines, AM More by this author
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Journal:
British journal of cancer
Volume:
72
Issue:
6
Pages:
1364-1372
Publication date:
1995-12-05
DOI:
EISSN:
1532-1827
ISSN:
0007-0920
URN:
uuid:7021430e-1ee7-44ea-bd89-23a20d7b4b27
Source identifiers:
171613
Local pid:
pubs:171613

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