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Fine tuning of phosphorothioate inclusion in 2'-O-methyl oligonucleotides contributes to specific cell targeting for splice-switching modulation

Abstract:

Splice-switching antisense oligonucleotide- (SSO-) mediated correction of framedisrupting mutation-containing premessenger RNA (mRNA) transcripts using exon skipping is a highly promising treatment method for muscular diseases such as Duchenne muscular dystrophy (DMD). Phosphorothioate (PS) chemistry, a commonly used oligonucleotide modification, has been shown to increase the stability of and improve the pharmacokinetics of SSOs. However, the effect of PS inclusion in 2'-O-methyl SSOs (2OMe)...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.3389/fphys.2021.689179

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Clinical Neurosciences
Role:
Author
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Role:
Author
ORCID:
0000-0002-7131-2998
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Role:
Author
ORCID:
0000-0003-4729-4205
Publisher:
Frontiers Media
Journal:
Frontiers in Physiology More from this journal
Volume:
12
Article number:
689179
Place of publication:
Switzerland
Publication date:
2021-10-13
Acceptance date:
2021-09-06
DOI:
EISSN:
1664-042X
ISSN:
1664-042X
Pmid:
34721051
Language:
English
Keywords:
Pubs id:
1207792
Local pid:
pubs:1207792
Deposit date:
2023-09-21

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