A point mutation within CD45 exon A is the cause of variant CD45RA splicing in humans.

Journal article

The leukocyte common antigen (CD45) is alternatively spliced, generating various isoforms expressed on hemopoietic cells. The splicing pattern of CD45 in T cells is altered in some individuals who show abnormal expression of high molecular weight isoforms containing exon A. The variant splicing pattern was shown to be associated with heterozygosity for a silent point mutation within CD45 exon A. This C to G transition is located 77 nucleotides downstream of the splice acceptor junction of exo...

Expand abstract

Authors: Zilch, C; Walker, A; Timón, M; Goff, L; Wallace, D

• Journal: European journal of immunology
• Volume: 28
• Issue: 1
• Pages: 22-29
• Publication date: 1998-01-01
• DOI: 10.1002/(sici)1521-4141(199801)28:01<22::aid-immu22>3.0.co;2-7
• EISSN: 1521-4141
• ISSN: 0014-2980
• Language: English
• Keywords: Hybrid Cells; COS Cells; CHO Cells; RNA, Messenger; Antigens, CD45; Exons; Polymerase Chain Reaction; T-Lymphocytes; Molecular Weight; Humans; RNA Splicing; Mice; Genes, Synthetic; Cricetinae; Transfection; Point Mutation; Animals; Mutagenesis, Site-Directed; Alleles; Hela Cells; Cricetulus