In vivo gene transfer into the testis by electroporation and viral infection--a novel way to study testis and sperm function.

Journal article

The study of gene function in testis and sperm has been greatly assisted by creation of transgenic mice by injection of a transgene into the fertilised egg. However this approach is costly and laborious and is not applicable to other species of importance for the study of sperm function, such as the hamster. We have investigated alternative ways of expressing transgenes in mouse and hamster testis and sperm by in vivo gene transfer. DNA expression constructs were introduced into the testis by...

Expand abstract

Authors: Parrington, J; Coward, K; Hibbitt, O; Kubota, H; Young, C

• Journal: Society of Reproduction and Fertility supplement
• Volume: 65
• Pages: 469-474
• Publication date: 2007-01-01
• ISSN: 1747-3403
• Language: English
• Keywords: Green Fluorescent Proteins; Fluorescent Dyes; Models, Animal; Testis; Animals; Microscopy, Fluorescence; Transduction, Genetic; Genetic Vectors; Gene Expression; Mice; Lentivirus; Male; Spermatozoa; Electroporation; Mesocricetus; DNA; Cricetinae; Mice, Inbred Strains