Single-molecule FRET reveals a corkscrew RNA structure for the polymerase-bound influenza virus promoter.

Journal article

The influenza virus is a major human and animal pathogen responsible for seasonal epidemics and occasional pandemics. The genome of the influenza A virus comprises eight segments of single-stranded, negative-sense RNA with highly conserved 5' and 3' termini. These termini interact to form a double-stranded promoter structure that is recognized and bound by the viral RNA-dependent RNA polymerase (RNAP); however, no 3D structural information for the influenza polymerase-bound promoter exists. F...

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Authors: Tomescu, A; Robb, N; Hengrung, N; Fodor, E; Kapanidis, A

• Publication status: Published
• Publisher: National Academy of Sciences
• Journal: Proceedings of the National Academy of Sciences of the United States of America
• Volume: 111
• Issue: 32
• Pages: E3335-E3342
• Publication date: 2014-08-01
• DOI: 10.1073/pnas.1406056111
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: Viral Proteins; Humans; RNA, Viral; RNA Replicase; Promoter Regions, Genetic; Binding Sites; Nucleic Acid Conformation; Fluorescence Resonance Energy Transfer; Models, Molecular; Influenza A Virus, H3N2 Subtype; Animals