Medium-throughput production of recombinant human proteins: Protein production in insect cells

Journal article

This chapter describes the step-by-step methods employed by the Structural Genomics Consortium (SGC) for screening and producing proteins in the baculovirus expression vector system (BEVS). This eukaryotic expression system was selected and a screening process established in 2007 as a measure to tackle the more challenging kinase, RNA-DNA processing and integral membrane protein families on our target list. Here, we discuss our platform for identifying soluble proteins from 3 ml of insect cel...

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Authors: Mahajan, P; Strain-Damerell, C; Gileadi, O; Burgess-Brown, N

• Journal: Methods in Molecular Biology
• Volume: 1091
• Pages: 95-121
• Publication date: 2014-01-01
• DOI: 10.1007/978-1-62703-691-7-6
• ISSN: 1064-3745
• Language: English
• Keywords: Expression; SEC; BEVS; Purification; Baculovirus; IMAC; Insect cells; Gel filtration; Protein; Recombinant