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Medium-throughput production of recombinant human proteins: Protein production in insect cells

Abstract:

This chapter describes the step-by-step methods employed by the Structural Genomics Consortium (SGC) for screening and producing proteins in the baculovirus expression vector system (BEVS). This eukaryotic expression system was selected and a screening process established in 2007 as a measure to tackle the more challenging kinase, RNA-DNA processing and integral membrane protein families on our target list. Here, we discuss our platform for identifying soluble proteins from 3 ml of insect cel...

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Publisher copy:
10.1007/978-1-62703-691-7-6

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Genomics Consortium
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Role:
Author
Journal:
Methods in Molecular Biology More from this journal
Volume:
1091
Pages:
95-121
Publication date:
2014-01-01
DOI:
ISSN:
1064-3745
Language:
English
Keywords:
Pubs id:
pubs:447292
UUID:
uuid:6d214994-407b-4f5a-b7cc-27c41579bf89
Local pid:
pubs:447292
Source identifiers:
447292
Deposit date:
2014-06-18

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