Fast measurement of sarcomere length and cell orientation in Langendorff-perfused hearts using remote focusing microscopy.
RATIONALE: Sarcomere length (SL) is a key indicator of cardiac mechanical function, but current imaging technologies are limited in their ability to unambiguously measure and characterize SL at the cell level in intact, living tissue. OBJECTIVE: We developed a method for measuring SL and regional cell orientation using remote focusing microscopy, an emerging imaging modality that can capture light from arbitrary oblique planes within a sample. METHODS AND RESULTS: We present a protocol that u...Expand abstract
- Publication status:
- Publisher copy:
- Copyright date: