Journal article
High spatial and temporal resolution Ca2+ imaging of myocardial strips from human, pig and rat
- Abstract:
-
Ca2+ handling within cardiac myocytes underpins coordinated contractile function within the beating heart. This protocol enables high spatial and temporal Ca2+ imaging of ex vivo multicellular myocardial strips. The endocardial surface is retained, and strips of 150–300-µm thickness are dissected, loaded with Ca2+ indicators and mounted within 1.5 h. A list of the equipment and reagents used and the key methodological aspects allowing the use of this technique on strips from any chamber of th...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Authors
Funding
Bibliographic Details
- Publisher:
- Springer Nature Publisher's website
- Journal:
- Nature Protocols Journal website
- Volume:
- 16
- Pages:
- 4650–4675
- Publication date:
- 2021-08-16
- Acceptance date:
- 2021-06-14
- DOI:
- EISSN:
-
1750-2799
- ISSN:
-
1754-2189
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
1190771
- Local pid:
- pubs:1190771
- Deposit date:
- 2021-08-13
Terms of use
- Copyright holder:
- Boysova et al.
- Copyright date:
- 2021
- Rights statement:
- © The Author(s) 2021
- Notes:
- This is the accepted manuscript version of the article. The final version is available online from Springer Nature at: https://doi.org/10.1038/s41596-021-00590-6
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