The application of single-cell RNA sequencing in vaccinology

Journal article : Review

Single-cell RNA sequencing allows highly detailed profiling of cellular immune responses from limited-volume samples, advancing prospects of a new era of systems immunology. The power of single-cell RNA sequencing offers various opportunities to decipher the immune response to infectious diseases and vaccines. Here, we describe the potential uses of single-cell RNA sequencing methods in prophylactic vaccine development, concentrating on infectious diseases including COVID-19. Using examples from several diseases, we review how single-cell RNA sequencing has been used to evaluate the immunological response to different vaccine platforms and regimens. By highlighting published and unpublished single-cell RNA sequencing studies relevant to vaccinology, we discuss some general considerations how the field could be enriched with the widespread adoption of this technology.

Authors: Noé, A; Cargill, TN; Nielsen, CM; Russell, AJC; Barnes, E

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Hindawi
• Journal: Journal of Immunology Research
• Volume: 2020
• Article number: 8624963
• Publication date: 2020-08-06
• Acceptance date: 2020-07-09
• DOI: 10.1155/2020/8624963
• EISSN: 2314-7156
• ISSN: 2314-8861
• Pmid: 32802896
• Language: English
• Keywords: FFR
• Subtype: Review