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New vector for transfer of yeast artificial chromosomes to mammalian cells.

Abstract:

A modification vector has been constructed to facilitate the transfer of yeast artificial chromosomes (YACs) to mammalian cells in culture by targeting a dominant selectable marker (G418 resistance) to the right arm of pYAC4 clones. The ADE2 gene is used for yeast selection with consequent disruption of the URA3 gene, allowing direct modification of YACs within the common host strain AB1380, and providing a simple test for correct targeting. This vector has been tested by modification of a 55...

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Publication status:
Published

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Publisher copy:
10.1007/bf01233531

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Human Genetics Wt Centre
Role:
Author
Journal:
Somatic cell and molecular genetics More from this journal
Volume:
19
Issue:
2
Pages:
161-169
Publication date:
1993-03-01
DOI:
EISSN:
1572-9931
ISSN:
0740-7750
Language:
English
Keywords:
Pubs id:
pubs:71858
UUID:
uuid:6ad14dcf-a780-4407-8746-de297273442e
Local pid:
pubs:71858
Source identifiers:
71858
Deposit date:
2012-12-19

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