Journal article
Activation mechanism of ATP-sensitive K+ channels explored with real-time nucleotide binding
- Abstract:
-
The response of ATP-sensitive K+ channels (KATP) to cellular metabolism is coordinated by three classes of nucleotide binding site (NBS). We used a novel approach involving labeling of intact channels in a native, membrane environment with a non-canonical fluorescent amino acid and measurement (using FRET with fluorescent nucleotides) of steady-state and time-resolved nucleotide binding to dissect the role of NBS2 of the accessory SUR1 subunit of KATP in channel gating. Binding to NBS2 was Mg...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Authors
Funding
Bibliographic Details
- Publisher:
- eLife Sciences Publisher's website
- Journal:
- eLife Journal website
- Volume:
- 8
- Issue:
- 2019
- Article number:
- e41103
- Publication date:
- 2019-02-21
- Acceptance date:
- 2019-02-14
- DOI:
- EISSN:
-
2050-084X
- ISSN:
-
2050-084X
- Pmid:
-
30789344
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:980975
- UUID:
-
uuid:6a9f7194-fe39-4c57-8dc9-f094863004f0
- Local pid:
- pubs:980975
- Source identifiers:
-
980975
- Deposit date:
- 2019-04-02
Terms of use
- Copyright holder:
- Puljung et al
- Copyright date:
- 2019
- Notes:
- © The Authors. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited
- Licence:
- CC Attribution (CC BY)
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