Selection and analysis of a mutant cell line defective in the hypoxia-inducible factor-1 alpha-subunit (HIF-1alpha). Characterization of hif-1alpha-dependent and -independent hypoxia-inducible gene expression.

Journal article

Hypoxia-inducible expression has been demonstrated for many groups of mammalian genes, and studies of transcriptional control have revealed the existence of hypoxia-responsive elements (HREs) in the cis-acting sequences of several of these genes. These sequences generally contain one or more binding sites for a heterodimeric DNA binding complex termed hypoxia-inducible factor-1 (HIF-1). To analyze this response further, Chinese hamster ovary cells were stably transfected with plasmids bearing...

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Authors: Wood, S; Wiesener, MS; Yeates, K; Okada, N; Pugh, C

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 273
• Issue: 14
• Pages: 8360-8368
• Publication date: 1998-04-01
• DOI: 10.1074/jbc.273.14.8360
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Nuclear Proteins; Cell Hypoxia; Hypoxia-Inducible Factor 1; Cricetinae; Hypoxia-Inducible Factor 1, alpha Subunit; Mutation; DNA-Binding Proteins; Gene Expression Regulation; Transcription Factors; CHO Cells; Animals