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Absolute quantitation of disease protein biomarkers in a single LC-MS acquisition using apolipoprotein F as an example

Abstract:

LC-MS and immunoassay can detect protein biomarkers. Immunoassays are more commonly used but can potentially be outperformed by LC-MS. These techniques have limitations including the necessity to generate separate calibration curves for each biomarker. We present a rapid mass spectrometry-based assay utilising a universal calibration curve. For the first time we analyse clinical samples using the HeavyPeptide IGNIS kit which establishes a 6-point calibration curve and determines the biomarke...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1038/s41598-017-12229-2

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Department:
Oxford, MSD, Biochemistry
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Author
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Department:
Oxford, MSD, Biochemistry
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Author
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Department:
Oxford, MSD, NDM, NDM Experimental Medicine
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Author
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Department:
Merton College
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Author

Contributors

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Editor
Oxford Glycobiology Endowment More from this funder
United Therapeutics Corporation More from this funder
Publisher:
Nature Publishing Group Publisher's website
Journal:
Scientific Reports Journal website
Volume:
7
Issue:
12072
Pages:
1-9
Publication date:
2017-09-21
Acceptance date:
2017-09-06
DOI:
ISSN:
2045-2322
Pubs id:
pubs:725681
URN:
uri:690658a1-38e1-496b-a704-24e6f3dd3cea
UUID:
uuid:690658a1-38e1-496b-a704-24e6f3dd3cea
Local pid:
pubs:725681
Language:
English
Keywords:

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