Journal article
Single-molecule and super-resolution imaging of transcription in living bacteria
- Abstract:
- In vivo single-molecule and super-resolution techniques are transforming our ability to study transcription as it takes place in its native environment in living cells. This review will detail the methods for imaging single molecules in cells, and the data-analysis tools which can be used to extract quantitative information on the spatial organization, mobility, and kinetics of the transcription machinery from these experiments. Furthermore, we will highlight studies which have applied these techniques to shed new light on bacterial transcription.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 2.1MB, Terms of use)
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- Publisher copy:
- 10.1016/j.ymeth.2017.04.001
Authors
+ Engineering and Physical Sciences Research Council
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- Grant:
- University of Oxford LSI-DTC
+ Biotechnology and Biological Sciences Research Council
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- Grant:
- BB/J00054X/1
- BB/N018656/1
- Publisher:
- Elsevier
- Journal:
- Methods More from this journal
- Volume:
- 120
- Pages:
- 103-114
- Publication date:
- 2017-04-13
- Acceptance date:
- 2017-04-05
- DOI:
- EISSN:
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1095-9130
- ISSN:
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1046-2023
- Pmid:
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28414097
- Language:
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English
- Keywords:
- Pubs id:
-
pubs:691144
- UUID:
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uuid:68f81e77-eb6c-43b9-b2e0-af408c1a9582
- Local pid:
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pubs:691144
- Deposit date:
-
2018-02-27
Terms of use
- Copyright holder:
- M Stracy and AN Kapanidis
- Copyright date:
- 2017
- Notes:
- © 2017 The Authors. Published by Elsevier Inc.
- Licence:
- CC Attribution (CC BY)
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