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A simple in situ assay to assess plant-associative bacterial nitrogenase activity

Abstract:

Assessment of plant-associative bacterial nitrogen (N) fixation is crucial for selection and development of elite diazotrophic inoculants that could be used to supply cereal crops with nitrogen in a sustainable manner. Although diazotrophic bacteria possess diverse oxygen tolerance mechanisms, most require a sub 21% oxygen environment to achieve optimal stability and function of the N-fixing catalyst nitrogenase. Consequently, assessment of N fixation is routinely carried out on "free-living"...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.3389/fmicb.2021.690439

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Institution:
University of Oxford
Department:
PLANT SCIENCES
Sub department:
Plant Sciences
Role:
Author
ORCID:
0000-0001-5087-6455
Biotechnology and Biological Sciences Research Council More from this funder
Publisher:
Frontiers Media Publisher's website
Journal:
Frontiers in Microbiology Journal website
Volume:
12
Article number:
690439
Publication date:
2021-06-23
Acceptance date:
2021-05-25
DOI:
EISSN:
1664-302X
Pmid:
34248916
Language:
English
Keywords:
Pubs id:
1186656
Local pid:
pubs:1186656
Deposit date:
2021-09-20

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