Journal article
Voltammetric detection of glutathione; an adsorptive stripping voltammetry approach
- Abstract:
- A simple, sensitive, and rapid detection of glutathione by cyclic voltammetry using a bare glassy carbon electrode is reported in which glutathione forms a 1:1 complex compound with copper(II) ion. This complex compound is adsorbed onto the electrode surface and undergoes electrochemical oxidation at a characteristic oxidation potential of ca. - 0.20 V vs standard mercury/mercurous sulphate reference electrode, which is used to detect the glutathione concentration. The linear dynamic range is obtained for a glutathione concentration of 1 µM to 12.5 µM, and the sensitivity is found to be 0.1 ± 0.002 μA μM-1. The low limit of detection (n=3) of 0.14 μM and the precision of 1.8% are achieved using a simple, unmodified electrode. The robustness of the present methodology is demonstrated by the successful quantitative analysis of glutathione in the presence of cysteine.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Accepted manuscript, pdf, 673.4KB, Terms of use)
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- Publisher copy:
- 10.1039/C6AN00550K
Authors
+ European Research Council
More from this funder
- Funding agency for:
- Shimizu, K
- Grant:
- FP7/2007-2013) Marie Skłodowska Curie Intra-European Fellowship
- Publisher:
- Royal Society of Chemistry
- Journal:
- Analyst More from this journal
- Volume:
- 141
- Pages:
- 2904-2910
- Publication date:
- 2016-04-01
- Acceptance date:
- 2016-04-01
- DOI:
- EISSN:
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1364-5528
- ISSN:
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0003-2654
- Keywords:
- Pubs id:
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pubs:613087
- UUID:
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uuid:68c73a20-c08f-4fc7-b9ec-e514ab0a99d0
- Local pid:
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pubs:613087
- Source identifiers:
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613087
- Deposit date:
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2016-04-04
- ARK identifier:
Terms of use
- Copyright holder:
- Areias, Shimizu and Compton
- Copyright date:
- 2016
- Notes:
- Authors will always retain their copyright for articles published with the Royal Society of Chemistry. This is the accepted manuscript version of the article. The final version is available online from the Royal Society of Chemistry at: [10.1039/C6AN00550K]
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