P450(BM3) on steroids: the Swiss Army knife P450 enzyme just gets better.

Journal article

The catalytic regio- and stereoselective oxidation of aliphatic C-H bonds by using atmospheric dioxygen as the oxidant is a highly desirable reaction. Reetz and co-workers have recently reported the development of P450(BM3) mutants that oxidise testosterone with greatly increased turnover activity, total stereoselectivity and >95 % regioselectivity.

Authors: Wong, L

• Publication status: Published
• Journal: Chembiochem : a European journal of chemical biology
• Volume: 12
• Issue: 17
• Pages: 2537-2539
• Publication date: 2011-11-01
• DOI: 10.1002/cbic.201100606
• EISSN: 1439-7633
• ISSN: 1439-4227
• Language: English
• Keywords: Mutation; Testosterone; Biocatalysis; Stereoisomerism; NADPH-Ferrihemoprotein Reductase; Protein Structure, Secondary; Steroids; Cytochrome P-450 Enzyme System; Catalytic Domain; Oxidation-Reduction; Bacterial Proteins