Charge detection mass spectrometry on human-amplified fibrils from different synucleinopathies

Journal article

Amyloid fibrils are self-assembled mesoscopic protein aggregates, which can accumulate to form deposits or plaques in the brain. In vitro amplification of fibrils can be achieved with real-time quaking-induced conversion (RT-QuIC). However, this emerging technique would benefit from a complementary method to assess structural properties of the amplification products. This work demonstrates the feasibility of nanospray-charge-detection-mass-spectrometry (CDMS) performed on α-synuclein (αSyn) fibrils amplified from human brains with Parkinson's disease (PD) or Dementia with Lewy bodies (DLB) and its synergistic combination with RT-QuIC.

Authors: Tsirkou, A; Kaczorowski, F; Verdurand, M; Raffoul, R; Pansieri, J

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Royal Society of Chemistry
• Journal: Chemical Communications
• Volume: 58
• Issue: 51
• Pages: 7192-7195
• Publication date: 2022-06-23
• DOI: 10.1039/d2cc00200k
• EISSN: 1364-548X
• ISSN: 1359-7345
• Language: English
• Keywords: Chromatography; Mass spectrometry; Medicine; Parkinson's disease; Alpha-synuclein; Synucleinopathies; Neuropathology; Amyloid β; Pathology; Amyloid fibril; Chemistry; Biochemistry; Fibril