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Intact free prostate-specific antigen and free and total human glandular kallikrein 2. Elimination of assay interference by enzymatic digestion of antibodies to F(ab')2 fragments.

Abstract:

Various blood constituents can interfere with immunoassays, usually by binding the Fc portion of antibodies. Our previously developed assays for intact free prostate-specific antigen (PSA), free human kallikrein 2 (hK2), and total hK2 frequently yielded falsely high results despite including an excess of scavenger antibodies. We investigated whether this interference could be eliminated by replacing monoclonal capture or tracer antibodies with F(ab')2 or recombinant Fab fragments. Female hepa...

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Publisher copy:
10.1021/ac061201+

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Surgical Sciences
Role:
Author
Journal:
Analytical chemistry More from this journal
Volume:
78
Issue:
22
Pages:
7809-7815
Publication date:
2006-11-01
DOI:
EISSN:
1520-6882
ISSN:
0003-2700
Language:
English
Keywords:
Pubs id:
pubs:365952
UUID:
uuid:681bfaf5-dd5d-45cd-ba82-ee36ba2c0c86
Local pid:
pubs:365952
Source identifiers:
365952
Deposit date:
2013-11-16

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