Intact free prostate-specific antigen and free and total human glandular kallikrein 2. Elimination of assay interference by enzymatic digestion of antibodies to F(ab')2 fragments.

Journal article

Various blood constituents can interfere with immunoassays, usually by binding the Fc portion of antibodies. Our previously developed assays for intact free prostate-specific antigen (PSA), free human kallikrein 2 (hK2), and total hK2 frequently yielded falsely high results despite including an excess of scavenger antibodies. We investigated whether this interference could be eliminated by replacing monoclonal capture or tracer antibodies with F(ab')2 or recombinant Fab fragments. Female hepa...

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Authors: Väisänen, V; Peltola, M; Lilja, H; Nurmi, M; Pettersson, K

• Journal: Analytical chemistry
• Volume: 78
• Issue: 22
• Pages: 7809-7815
• Publication date: 2006-11-01
• DOI: 10.1021/ac061201+
• EISSN: 1520-6882
• ISSN: 0003-2700
• Language: English
• Keywords: Antibody Specificity; Male; Humans; Heparin; Tumor Markers, Biological; Immunoglobulin Fab Fragments; Antibodies, Monoclonal; Tissue Kallikreins; Prostate-Specific Antigen; Edetic Acid; Animals; Diagnosis, Differential; Prostatic Neoplasms; Immunoassay; Mice