Journal article
Evaluation of a human BCG challenge model to assess antimycobacterial immunity induced by BCG and a candidate TB vaccine, MVA85A, alone and in combination
- Abstract:
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Background. A new vaccine is urgently needed to combat tuberculosis. However, without a correlate of protection, selection of the vaccines to take forward into large-scale efficacy trials is difficult. Use of bacille Calmette-Guérin (BCG) as a surrogate for human Mycobacterium tuberculosis challenge is a novel model that could aid selection.
Methods. Healthy adults were assigned to groups A and B (BCG-naive) or groups C and D (BCG-vaccinated). Groups B and D received candidate tuberculosis vaccine MVA85A. Participants were challenged with intradermal BCG 4 weeks after those who received MVA85A. Skin biopsies of the challenge site were taken 2 weeks post challenge and BCG load quantified by culture and quantitative polymerase chain reaction (qPCR).
Results. Volunteers with a history of BCG showed some degree of protective immunity to challenge, having lower BCG loads compared with volunteers without prior BCG, regardless of MVA85A status. There was a significant inverse correlation between antimycobacterial immunity at peak response after MVA85A and BCG load detected by qPCR.
Conclusion. Our results support previous findings that this BCG challenge model is able to detect differences in antimycobacterial immunity induced by vaccination and could aid in the selection of candidate tuberculosis vaccines for field efficacy testing.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(bin, 536.4KB, Terms of use)
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- Publisher copy:
- 10.1093/infdis/jit647
Authors
- Publisher:
- Oxford University Press
- Journal:
- Journal of Infectious Diseases More from this journal
- Publication date:
- 2013-11-01
- Edition:
- Publisher's version
- DOI:
- EISSN:
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1537-6613
- ISSN:
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0022-1899
- Language:
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English
- Keywords:
- Subjects:
- UUID:
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uuid:679ae149-f9c7-4b72-b1a2-bc977a4362af
- Local pid:
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ora:8052
- Deposit date:
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2014-02-24
- ARK identifier:
Terms of use
- Copyright holder:
- Harris et al
- Copyright date:
- 2013
- Notes:
- This is an Advance Access article. © 2013 Harris et al. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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