Evaluation of a monoclonal antibody blocking ELISA for the detection of group-specific antibodies to bluetongue virus in experimental and field sera.

Journal article

In order to overcome serological cross-reactions among orbivirus serogroups, which can hinder the accurate diagnosis of bluetongue virus (BTV) infection of livestock, a blocking ELISA (B-ELISA) incorporating a monoclonal antibody (20E9B7G2) with specificity for the BTV serogroup was developed. Experimental antisera raised to South African BTV serotypes 1 to 19 were tested in the B-ELISA and all blocked the binding of 20E9B7G2 to BTV antigen. The sensitivity and specificity of the assay was ev...

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Authors: Lunt, R; White, JR; Blacksell, S

• Publication status: Published
• Journal: Journal of general virology
• Volume: 69 ( Pt 11)
• Issue: 11
• Pages: 2729-2740
• Publication date: 1988-11-01
• DOI: 10.1099/0022-1317-69-11-2729
• EISSN: 1465-2099
• ISSN: 0022-1317
• Language: English
• Keywords: Antibodies, Monoclonal; Bluetongue; Bluetongue virus; Antibodies, Viral; Reoviridae; Enzyme-Linked Immunosorbent Assay; Time Factors; Sheep; Animals; Cattle