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Fluorescence correlation spectroscopy with a total internal reflection fluorescence STED microscope (TIRF-STED-FCS).

Abstract:

We characterize a novel fluorescence microscope which combines the high spatial discrimination of a total internal reflection epi-fluorescence (epi-TIRF) microscope with that of stimulated emission depletion (STED) nanoscopy. This combination of high axial confinement and dynamic-active lateral spatial discrimination of the detected fluorescence emission promises imaging and spectroscopy of the structure and function of cell membranes at the macro-molecular scale. Following a full theoretical...

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Publisher copy:
10.1364/oe.20.005243

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
Publisher:
Optical Society of American (OSA)
Journal:
Optics express More from this journal
Volume:
20
Issue:
5
Pages:
5243-5263
Publication date:
2012-02-01
DOI:
EISSN:
1094-4087
Language:
English
Keywords:
Pubs id:
pubs:316364
UUID:
uuid:66ed52d9-6a4d-4841-9efc-ba4aea9eaf4f
Local pid:
pubs:316364
Source identifiers:
316364
Deposit date:
2013-11-16

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