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A versatile spectrophotometric protein tyrosine phosphatase assay based on 3-nitrophosphotyrosine containing substrates.

Abstract:

A versatile assay for protein tyrosine phosphatases (PTP) employing 3-nitrophosphotyrosine containing peptidic substrates is described. These therapeutically important phosphatases feature in signal transduction pathways. The assay involves spectrophotometric detection of 3-nitrotyrosine production from 3-nitrophosphotyrosine containing peptidic substrates, which are accepted by many PTPs. Compared to conventional chromogenic phosphate derivatives, the more realistic peptidic substrates allow...

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Publication status:
Published

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Publisher copy:
10.1016/j.ab.2013.11.023

Authors


van Ameijde, J More by this author
Overvoorde, J More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Genomics Consortium
den Hertog, J More by this author
Ruijtenbeek, R More by this author
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Journal:
Analytical biochemistry
Volume:
448
Issue:
1
Pages:
9-13
Publication date:
2014-03-05
DOI:
EISSN:
1096-0309
ISSN:
0003-2697
URN:
uuid:66506337-150e-4944-9054-436191300761
Source identifiers:
444312
Local pid:
pubs:444312

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