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A versatile spectrophotometric protein tyrosine phosphatase assay based on 3-nitrophosphotyrosine containing substrates.

Abstract:

A versatile assay for protein tyrosine phosphatases (PTP) employing 3-nitrophosphotyrosine containing peptidic substrates is described. These therapeutically important phosphatases feature in signal transduction pathways. The assay involves spectrophotometric detection of 3-nitrotyrosine production from 3-nitrophosphotyrosine containing peptidic substrates, which are accepted by many PTPs. Compared to conventional chromogenic phosphate derivatives, the more realistic peptidic substrates allow...

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Publication status:
Published

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Publisher copy:
10.1016/j.ab.2013.11.023

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Genomics Consortium
Role:
Author
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Journal:
Analytical biochemistry
Volume:
448
Issue:
1
Pages:
9-13
Publication date:
2014-03-01
DOI:
EISSN:
1096-0309
ISSN:
0003-2697
Source identifiers:
444312
Language:
English
Keywords:
Pubs id:
pubs:444312
UUID:
uuid:66506337-150e-4944-9054-436191300761
Local pid:
pubs:444312
Deposit date:
2014-02-07

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