Journal article
A versatile spectrophotometric protein tyrosine phosphatase assay based on 3-nitrophosphotyrosine containing substrates.
- Abstract:
-
A versatile assay for protein tyrosine phosphatases (PTP) employing 3-nitrophosphotyrosine containing peptidic substrates is described. These therapeutically important phosphatases feature in signal transduction pathways. The assay involves spectrophotometric detection of 3-nitrotyrosine production from 3-nitrophosphotyrosine containing peptidic substrates, which are accepted by many PTPs. Compared to conventional chromogenic phosphate derivatives, the more realistic peptidic substrates allow...
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- Publication status:
- Published
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Bibliographic Details
- Journal:
- Analytical biochemistry
- Volume:
- 448
- Issue:
- 1
- Pages:
- 9-13
- Publication date:
- 2014-03-01
- DOI:
- EISSN:
-
1096-0309
- ISSN:
-
0003-2697
- Source identifiers:
-
444312
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:444312
- UUID:
-
uuid:66506337-150e-4944-9054-436191300761
- Local pid:
- pubs:444312
- Deposit date:
- 2014-02-07
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- Copyright date:
- 2014
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