Membrane protein stoichiometry determined from the step-wise photobleaching of dye-labelled subunits.

Journal article

Body count.In a generally applicable approach, the number of subunits in fluorescently-labelled protein complexes has been determined by counting photobleaching steps from individual molecules (see figure). The distribution of steps in the pore-forming toxins α-haemolysin and leukocidin indicate seven subunits for α-hemolysin, and four LukF and four LukS subunits for leukocidin. (Graph Presented). © 2007 Wiley-VCH Verlag GmbHandCo. KGaA.

Authors: Das, S; Darshi, M; Cheley, S; Wallace, M; Bayley, H

• Publication status: Published
• Journal: Chembiochem : a European journal of chemical biology
• Volume: 8
• Issue: 9
• Pages: 994-999
• Publication date: 2007-06-01
• DOI: 10.1002/cbic.200600474
• EISSN: 1439-7633
• ISSN: 1439-4227
• Language: English
• Keywords: Crystallography, X-Ray; Microscopy, Fluorescence; Electrophoresis, Polyacrylamide Gel; Photobleaching; Membrane Proteins; Coloring Agents; Transcription, Genetic; Hemolysis; Kinetics; Animals; Rabbits; Protein Biosynthesis; Fluorescent Dyes; Erythrocyte Membrane; Leukocidins