Journal article
Open circuit potential as a tool for the assessment of binding kinetics and reagentless protein quantitation
- Abstract:
- A microfluidic open circuit potential label-free protein assay was developed for the reagentless quantification of C-reactive protein (CRP), a model protein target, and further utilized to assess target-receptor binding kinetics. Generated sensors have very high baseline stabilities (<1% change in 100 min) and high levels of selectivity in complex media. Real-time assays are fast (<20 min), of high sensitivity (1 ng/mL limit of detection for CRP in serum), and resolve kinetic and thermodynamic characteristics that correlate well with those resolved optically. The assay shows excellent correlation with an enzyme-linked immunosorbent assay analysis of patient samples. The methodology has value in potentially underpinning a low-cost, rapid, and sensitive single-step biomarker quantification.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
-
-
(Preview, Accepted manuscript, pdf, 861.6KB, Terms of use)
-
- Publisher copy:
- 10.1021/acs.analchem.1c03292
Authors
- Publisher:
- American Chemical Society
- Journal:
- Analytical Chemistry More from this journal
- Volume:
- 93
- Issue:
- 44
- Pages:
- 14748-14754
- Publication date:
- 2021-10-26
- Acceptance date:
- 2021-10-10
- DOI:
- EISSN:
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1520-6882
- ISSN:
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0003-2700
- Pmid:
-
34699180
- Language:
-
English
- Keywords:
- Pubs id:
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1205985
- Local pid:
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pubs:1205985
- Deposit date:
-
2021-12-22
- ARK identifier:
Terms of use
- Copyright holder:
- American Chemical Society
- Copyright date:
- 2021
- Rights statement:
- Copyright © 2021 American Chemical Society.
- Notes:
- This is the accepted manuscript version of the article. The final version is available online from the American Chemical Society at: https://doi.org/10.1021/acs.analchem.1c03292
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