Journal article

Monitoring protein-metal binding by 19F NMR – a case study with the New Delhi metallo-β-lactamase 1

Abstract:: 19F NMR protein observed spectroscopy is evaluated as a method for analysing protein metal binding using the New Delhi metallo-β-lactamase 1. The results imply 19F NMR is useful for analysis of different metallated protein states and investigations on equilibrium states in the presence of inhibitors. One limitation is that 19F labelling may affect metal ion binding. The sensitive readout of changes in protein behaviour observed by 19F NMR spectra coupled with the broad scope of tolerated conditions (e.g. buffer variations) means 19F NMR should be further investigated for studying metal ion interactions and the inhibition of metallo-enzymes during drug discovery.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

GOWAR, M. Z. A. H. N., Rydzik, A. M., Brem, J., Chandler, S. A., Benesch, J. L. P., Claridge, T. D. W., & Schofield, C. J. (2020). Monitoring protein-metal binding by 19F NMR – a case study with the New Delhi metallo-β-lactamase 1. RSC Medicinal Chemistry, 11(3), 387–391.

MLA Style

GOWAR, Mrs Z. AH N., et al. “Monitoring Protein-Metal Binding by 19F NMR – a Case Study with the New Delhi Metallo-β-Lactamase 1.” RSC Medicinal Chemistry, vol. 11, no. 3, Royal Society of Chemistry, 2020, pp. 387–91.

Chicago Style

GOWAR, Mrs ZAHN, AM Rydzik, J Brem, SA Chandler, JLP Benesch, TDW Claridge, and CJ Schofield. 2020. “Monitoring Protein-Metal Binding by 19F NMR – a Case Study with the New Delhi Metallo-β-Lactamase 1.” RSC Medicinal Chemistry 11 (3): 387–91.
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Files:: Rydzik_et_al_Monitoring_protein-metal.pdf

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Publisher copy:: 10.1039/c9md00416e

Authors

+ Mrs ZAHN GOWAR More by this author

Role:: Author

+ Rydzik, AM More by this author

Institution:: University of Oxford
Division:: MPLS
Department:: Chemistry
Sub department:: Organic Chemistry
Role:: Author

+ Brem, J More by this author

Institution:: University of Oxford
Division:: MPLS
Department:: Chemistry
Sub department:: Organic Chemistry
Role:: Author
ORCID:: 0000-0002-0137-3226

+ Chandler, SA More by this author

Institution:: University of Oxford
Division:: MPLS
Department:: Chemistry
Sub department:: Organic Chemistry
Role:: Author

+ Benesch, JLP More by this author

Institution:: University of Oxford
Division:: MPLS
Department:: Chemistry
Sub department:: Organic Chemistry
Role:: Author

More authors...

Publisher:: Royal Society of Chemistry
Journal:: RSC Medicinal Chemistry More from this journal
Volume:: 11
Issue:: 3
Pages:: 387-391
Publication date:: 2020-02-21
Acceptance date:: 2019-12-31
DOI:: 10.1039/c9md00416e
EISSN:: 2632-8682

Language:: English
Keywords:: FFR
Pubs id:: 1090042
Local pid:: pubs:1090042
Deposit date:: 2020-03-30

Terms of use

Copyright holder:: Royal Society of Chemistry
Copyright date:: 2020
Rights statement:: © The Royal Society of Chemistry 2020. Open Access Article: This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Licence:: CC Attribution (CC BY)

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