A structural basis for the interaction of urea with lysozyme.

Journal article

The effect of urea on the crystal structure of hen egg-white lysozyme has been investigated using X-ray crystallography. High resolution structures have been determined from crystals grown in the presence of 0, 0.7, 2, 3, 4, and 5 M urea and from crystals soaked in 9 M urea. All the forms are essentially isomorphous with the native type II crystals, and the derived structures exhibit excellent geometry and RMS differences from ideality in bond distances and angles. Comparison of the urea comp...

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Authors: Pike, A; Acharya, K

• Publication status: Published
• Journal: Protein science : a publication of the Protein Society
• Volume: 3
• Issue: 4
• Pages: 706-710
• Publication date: 1994-04-01
• DOI: 10.1002/pro.5560030419
• EISSN: 1469-896X
• ISSN: 0961-8368
• Language: English
• Keywords: Amino Acid Sequence; Crystallization; Muramidase; Crystallography, X-Ray; Models, Molecular; Urea; Molecular Sequence Data; Protein Conformation; Molecular Structure