- Abstract:
-
Knockout collections are invaluable tools for studying model organisms such as yeast. However, there are no large-scale knockout collections of human cells. Using gene-trap mutagenesis in near-haploid human cells, we established a platform to generate and isolate individual 'gene-trapped cells' and used it to prepare a collection of human cell lines carrying single gene-trap insertions. In most cases, the insertion can be reversed. This growing library covers 3,396 genes, one-third of the exp...
Expand abstract - Publisher copy:
- 10.1038/nmeth.2609
-
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- Journal:
- Nature methods
- Volume:
- 10
- Issue:
- 10
- Pages:
- 965-971
- Publication date:
- 2013-10-05
- DOI:
- EISSN:
-
1548-7105
- ISSN:
-
1548-7091
- URN:
-
uuid:64ba4455-e427-49bf-9273-12e27fa7eaa8
- Source identifiers:
-
474175
- Local pid:
- pubs:474175
- Language:
- English
- Keywords:
- Copyright date:
- 2013
Journal article
A reversible gene trap collection empowers haploid genetics in human cells.
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