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A reversible gene trap collection empowers haploid genetics in human cells.

Abstract:

Knockout collections are invaluable tools for studying model organisms such as yeast. However, there are no large-scale knockout collections of human cells. Using gene-trap mutagenesis in near-haploid human cells, we established a platform to generate and isolate individual 'gene-trapped cells' and used it to prepare a collection of human cell lines carrying single gene-trap insertions. In most cases, the insertion can be reversed. This growing library covers 3,396 genes, one-third of the exp...

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Publisher copy:
10.1038/nmeth.2609

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Journal:
Nature methods
Volume:
10
Issue:
10
Pages:
965-971
Publication date:
2013-10-01
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
Source identifiers:
474175
Language:
English
Keywords:
Pubs id:
pubs:474175
UUID:
uuid:64ba4455-e427-49bf-9273-12e27fa7eaa8
Local pid:
pubs:474175
Deposit date:
2014-11-20

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