Definition of an efficient synthetic poly(A) site.

Journal article

We constructed and analyzed a synthetic poly(A) (SPA) site that was based on the highly efficient poly(A) signal of the rabbit beta-globin gene. By use of the SPA, we demonstrate that the minimum sequences required for efficient polyadenylation are the AATAAA sequence and a GT/T-rich sequence with the correct spacing of 22-23 nucleotides between them. When placed downstream of the poly(A) site of the human alpha 2-globin gene, the SPA is used exclusively. We predict that the SPA, with its mor...

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Authors: Levitt, N; Briggs, D; Gil, A; Proudfoot, N

• Publication status: Published
• Journal: Genes and development
• Volume: 3
• Issue: 7
• Pages: 1019-1025
• Publication date: 1989-07-01
• DOI: 10.1101/gad.3.7.1019
• EISSN: 1549-5477
• ISSN: 0890-9369
• Language: English
• Keywords: Exons; Introns; Restriction Mapping; Base Sequence; Molecular Sequence Data; Globins; DNA; Transcription, Genetic; RNA, Messenger; Exonucleases; Humans; RNA Splicing; Rabbits; Poly A; Animals; Nucleic Acid Hybridization