Book section
Isolation of Leishmania promastigote flagella
- Abstract:
- Eukaryotic flagella are conserved multifunctional organelles with roles in motility, intercellular interactions and signal transduction. Leishmania possess a single flagellum at all stages of their life cycle. Flagella of promastigote forms in the fly are long and motile, with a canonical 9+2 microtubule axoneme and an extra-axonemal paraflagellar rod (PFR). This protocol describes a simple method for the isolation of Leishmania mexicana promastigote flagella, optimised to yield intact flagella that retain both the cytoskeletal elements (9+2 axoneme and PFR) and the surrounding membrane. The isolated flagella and deflagellated cell bodies are suitable for analysis by electron microscopy, protein mass spectrometry and lipidomics.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Accepted manuscript, pdf, 602.7KB, Terms of use)
-
- Publisher copy:
- 10.1007/978-1-0716-0294-2_29
Authors
- Publisher:
- Humana Press
- Pages:
- 485-495
- Series:
- Methods in Molecular Biology
- Series number:
- 2116
- Publication date:
- 2020-03-28
- Acceptance date:
- 2019-04-25
- DOI:
- EISSN:
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1940-6029
- ISSN:
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1064-3745
- EISBN:
- 9781071602942
- ISBN:
- 9781071602935
- Language:
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English
- Keywords:
- Pubs id:
-
pubs:994615
- UUID:
-
uuid:64161c05-567a-4797-a5bd-75f8523fc7c4
- Local pid:
-
pubs:994615
- Source identifiers:
-
994615
- Deposit date:
-
2019-05-15
- ARK identifier:
Terms of use
- Copyright holder:
- Springer Science+Business Media
- Copyright date:
- 2020
- Rights statement:
- © Springer Science+Business Media, LLC, part of Springer Nature 2020.
- Notes:
- This is the accepted manuscript version of the article. The final version is available online from Humana Press at: https://doi.org/10.1007/978-1-0716-0294-2_29
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