On the mechanism of loading the PCNA sliding clamp by RFC.

Journal article

Sliding clamps play central roles in a broad range of DNA replication and repair processes. The clamps form circular molecules that must be opened and resealed around DNA by the clamp loader complex to fulfil their function. While most eukaryotes and many archea possess a homo-trimeric PCNA, the PCNA of Sulfolobus solfataricus is a heterotrimer. Here, we exploit the asymmetry of S. solfataricus PCNA to create a series of circularly permuted PCNA subunit fusions, thereby covalently closing def...

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Authors: Dionne, I; Brown, N; Woodgate, R; Bell, S

• Publication status: Published
• Journal: Molecular microbiology
• Volume: 68
• Issue: 1
• Pages: 216-222
• Publication date: 2008-04-01
• DOI: 10.1111/j.1365-2958.2008.06150.x
• EISSN: 1365-2958
• ISSN: 0950-382X
• Language: English
• Keywords: Models, Biological; Protein Structure, Tertiary; DNA Replication; Protein Structure, Secondary; Proliferating Cell Nuclear Antigen; Protein Binding; Archaeal Proteins; Replication Protein C; Electrophoretic Mobility Shift Assay; Sulfolobus