Journal article
Oxidation of dietary linoleate occurs to a greater extent than dietary palmitate in vivo in humans
- Abstract:
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Background
It has been suggested that dietary polyunsaturated fatty acids (PUFA) are partitioned into oxidation pathways to a greater extent than dietary saturated fatty acids (SFA). Whilst this has been demonstrated in animal models, evidence in humans is lacking. The potential divergence in the metabolic fate of these dietary fatty acids (FA) may explain some of the reported differences in ectopic fat deposition with SFA and PUFA enriched diets.
Aims
To compare whole-body oxidation of dietary palmitate and linoleate after consumption of a single test meal.
Methods
In a randomized, crossover design 24 healthy volunteers (12 males and 12 females, matched for age and BMI) underwent two study days separated by 2-week washout period. During each study day participants consumed a standardized test meal which contained [U13C]palmitate or [U13C]linoleate. Blood and breath samples were collected over the 6 h postprandial period and the 13C enrichment in breath CO2 samples and plasma lipid fractions was determined.
Results
Appearance of 13C in expired CO2 was significantly (p < 0.05) increased after consumption of the meal containing [U13C]linoleate compared to the meal containing [U13C]palmitate. The recovery of tracer was 8.9 ± 1.2% [U13C]linoleate vs. 5.6 ± 0.4% [U13C]palmitate (p < 0.05). The incorporation of 13C from [U13C]palmitate was greater in plasma triacylglycerol and non-esterified fatty acids than [U13C]linoleate, whereas the incorporation of 13C from [U13C]linoleate was greater than [U13C]palmitate in plasma phospholipids. Although 13CO2 was significantly (p < 0.05) higher in females compared to males after consumption of [U13C]palmitate, there was no difference in 13CO2 between sexes after consumption of [U13C]linoleate.
Conclusions
We demonstrate that whole-body oxidation of dietary linoleate is comparatively higher than that of dietary palmitate in humans following consumption of a single mixed-test meal. We found indications of sexual dimorphism for dietary palmitate but not dietary linoleate.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Accepted manuscript, pdf, 422.6KB, Terms of use)
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- Publisher copy:
- 10.1016/j.clnu.2020.07.013
Authors
- Publisher:
- Elsevier
- Journal:
- Clinical Nutrition More from this journal
- Volume:
- 40
- Issue:
- 3
- Pages:
- 1108-1114
- Publication date:
- 2020-07-21
- Acceptance date:
- 2020-07-13
- DOI:
- ISSN:
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0261-5614
- Language:
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English
- Keywords:
- Pubs id:
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1119488
- Local pid:
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pubs:1119488
- Deposit date:
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2020-07-17
- ARK identifier:
Terms of use
- Copyright holder:
- Elsevier Ltd and European Society for Clinical Nutrition and Metabolism
- Copyright date:
- 2020
- Rights statement:
- © 2020 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.
- Notes:
- This is the accepted manuscript version of the article. The final version is available online from Elsevier at: https://doi.org/10.1016/j.clnu.2020.07.013
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