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Allosteric activation of an ion channel triggered by modification of mechanosensitive nano-pockets

Abstract:

Lipid availability within transmembrane nano-pockets of ion channels is linked with mechanosensation. However, the effect of hindering lipid-chain penetration into nano-pockets on channel structure has not been demonstrated. Here we identify nano-pockets on the large conductance mechanosensitive channel MscL, the high-pressure threshold channel. We restrict lipid-chain access to the nano-pockets by mutagenesis and sulfhydryl modification, and monitor channel conformation by PELDOR/DEER spectr...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41467-019-12591-x

Authors


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Role:
Author
ORCID:
0000-0002-0552-5784
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Role:
Author
ORCID:
0000-0003-2257-1595
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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
ORCID:
0000-0002-8204-7608
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Name:
Medical Research Council
Grant:
MR/M019152/1
Publisher:
Springer Nature
Journal:
Nature Communications More from this journal
Volume:
10
Issue:
1
Article number:
4619
Publication date:
2019-10-10
Acceptance date:
2019-09-17
DOI:
EISSN:
2041-1723
Pmid:
31601809
Language:
English
Keywords:
Pubs id:
1062804
Local pid:
pubs:1062804
Deposit date:
2020-06-15

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