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Benchmarking CRISPR-BP34 for point-of-care melioidosis detection in low-income and middle-income countries: a molecular diagnostics study

Abstract:

Background: Melioidosis is a neglected but often fatal tropical disease. The disease has broad clinical manifestations, which makes diagnosis challenging and time consuming. To improve diagnosis, we aimed to evaluate the performance of the CRISPR-Cas12a system (CRISPR-BP34) to detect Burkholderia pseudomallei DNA across clinical specimens from patients suspected to have melioidosis.

Methods: We conducted a prospective, ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/s2666-5247(23)00378-6

Authors


Publisher:
Elsevier
Journal:
Lancet Microbe More from this journal
Volume:
5
Issue:
4
Pages:
E379-E389
Publication date:
2024-03-13
Acceptance date:
2024-03-01
DOI:
EISSN:
2666-5247


Language:
English
Pubs id:
1875344
Local pid:
pubs:1875344
Deposit date:
2024-03-26
ARK identifier:

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