Measles virus transmembrane fusion protein synthesized de novo or presented in immunostimulating complexes is endogenously processed for HLA class I- and class II-restricted cytotoxic T cell recognition.

Journal article

The routes used by antigen-presenting cells (APC) to convert the transmembrane fusion glycoprotein (F) of measles virus (MV) to HLA class I and class II presentable peptides have been examined, using cloned cytotoxic T lymphocytes in functional assays. Presentation by Epstein-Barr virus-transformed B lymphoblastoid cell lines was achieved using live virus, ultraviolet light-inactivated virus, and purified MV-F delivered either as such or incorporated in immunostimulating complexes (MV-F-ISCOM). Only live virus and MV-F-ISCOM allow presentation by class I molecules, while all antigen preparations permit class II-restricted presentation. We observe presentation of MV-F from live virus and as MV-F-ISCOM by class II molecules in a fashion that is not perturbed by chloroquine. Our studies visualize novel presentation pathways of type I transmembrane proteins.

Authors: van Binnendijk, R; van Baalen, C; Poelen, M; de Vries, P; Boes, J

• Publication status: Published
• Journal: Journal of experimental medicine
• Volume: 176
• Issue: 1
• Pages: 119-128
• Publication date: 1992-07-01
• DOI: 10.1084/jem.176.1.119
• EISSN: 1540-9538
• ISSN: 0022-1007
• Language: English
• Keywords: T-Lymphocytes, Cytotoxic; Histocompatibility Antigens Class I; Animals; Viral Fusion Proteins; Antigen-Presenting Cells; Antigens, CD8; Histocompatibility Antigens Class II; Lymphocyte Activation; Humans; Measles virus; Antigens, CD4