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Dietary nitrogen alters codon bias and genome composition in parasitic microorganisms

Abstract:

Background: Genomes are composed of long strings of nucleotide monomers (A, C, G, and T) that are either scavenged from the organism’s environment or built from metabolic precursors. The biosynthesis of each nucleotide differs in atomic requirements with different nucleotides requiring different quantities of nitrogen atoms. However the impact of the relative availability of dietary nitrogen on genome composition and codon bias is poorly understood.

Results: Here we show that differential nitrogen availability, due to differences in environment and dietary inputs, is a major determinant of genome nucleotide composition and synonymous codon use in both bacterial and eukaryotic microorganisms. Specifically low nitrogen availability species use nucleotides that require fewer nitrogen atoms to encode the same genes when compared to high nitrogen availability species. Furthermore we provide a novel selection-mutation framework for the evaluation of the impact of metabolism on gene sequence evolution and show that it is possible to predict the metabolic inputs of related organisms from an analysis of the raw nucleotide sequence of their genes.

Conclusions: Taken together, these results reveal a previously hidden relationship between cellular metabolism and genome evolution and provide new insight into how genome sequence evolution can be influenced by adaptation to different diets and environment.

Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1186/s13059-016-1087-9

Authors


More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Plant Sciences
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Plant Sciences
Role:
Author


More from this funder
Funding agency for:
Kelly, S
Grant:
University Research Fellow
More from this funder
Grant:
Horizon 702 2020 no 637765


Publisher:
BioMed Central
Journal:
Genome Biology More from this journal
Volume:
17
Pages:
226
Publication date:
2016-11-01
Acceptance date:
2016-10-03
DOI:
ISSN:
1465-6906


Keywords:
Pubs id:
pubs:652540
UUID:
uuid:602d9e3d-cb8c-4e62-a656-884d117ad647
Local pid:
pubs:652540
Source identifiers:
652540
Deposit date:
2016-10-14

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