Journal article
Surface-enhanced Raman spectroscopy combined with stable isotope probing to monitor nitrogen assimilation at both bulk and single-cell level
- Abstract:
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Microbe-mediated biogeochemical cycle of nitrogen is a critical process in the environment. In this study, surface-enhanced Raman spectroscopy combined with 15N stable isotope probing (SERS-15N SIP) was developed as a new, non-destructive, and robust approach to probe nitrogen assimilation by bacteria at both bulk and single-cell level, and from pure culture to environmental microbial community. Multiple distinguishable SERS band shifts were observed and displayed a linear relationship with 1...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Accepted manuscript, pdf, 1.2MB)
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- Publisher copy:
- 10.1021/acs.analchem.6b04913
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Authors
Funding
+ Engineering and Physical Sciences Research Council
More from this funder
Funding agency for:
Huang, W
Grant:
NE/M002934/1
+ Natural Environment Research Council
More from this funder
Funding agency for:
Huang, W
Grant:
NE/M002934/1
China Scholarship Council
More from this funder
Bibliographic Details
- Publisher:
- American Chemical Society Publisher's website
- Journal:
- Analytical Chemistry Journal website
- Volume:
- 89
- Issue:
- 11
- Pages:
- 5793–5800
- Publication date:
- 2017-04-01
- Acceptance date:
- 2017-04-28
- DOI:
- EISSN:
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1520-6882
- ISSN:
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0003-2700
- Source identifiers:
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692072
Item Description
- Keywords:
- Pubs id:
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pubs:692072
- UUID:
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uuid:5fca75c2-6bbc-4f71-928e-553b420ddbe1
- Local pid:
- pubs:692072
- Deposit date:
- 2017-05-02
Terms of use
- Copyright holder:
- American Chemical Society
- Copyright date:
- 2017
- Notes:
- © 2017 American Chemical Society. This is the accepted manuscript version of the article. The final version is available online from American Chemical Society at: http://dx.doi.org/10.1021/acs.analchem.6b04913
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