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Surface-enhanced Raman spectroscopy combined with stable isotope probing to monitor nitrogen assimilation at both bulk and single-cell level

Abstract:

Microbe-mediated biogeochemical cycle of nitrogen is a critical process in the environment. In this study, surface-enhanced Raman spectroscopy combined with 15N stable isotope probing (SERS-15N SIP) was developed as a new, non-destructive, and robust approach to probe nitrogen assimilation by bacteria at both bulk and single-cell level, and from pure culture to environmental microbial community. Multiple distinguishable SERS band shifts were observed and displayed a linear relationship with 1...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted manuscript

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Publisher copy:
10.1021/acs.analchem.6b04913

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Department:
Oxford, MPLS, Engineering Science
Role:
Author
China Scholarship Council More from this funder
Publisher:
American Chemical Society Publisher's website
Journal:
Analytical Chemistry Journal website
Volume:
89
Issue:
11
Pages:
5793–5800
Publication date:
2017-04-05
Acceptance date:
2017-04-28
DOI:
EISSN:
1520-6882
ISSN:
0003-2700
Pubs id:
pubs:692072
URN:
uri:5fca75c2-6bbc-4f71-928e-553b420ddbe1
UUID:
uuid:5fca75c2-6bbc-4f71-928e-553b420ddbe1
Local pid:
pubs:692072

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