Three-dimensional (13)C-spectroscopic imaging in the isolated infarcted rat heart.

Journal article

Acquisition weighted (13)C-spectroscopic imaging with three spatial dimensions is demonstrated in the isolated, perfused rat heart. Experiments were performed at 11.75 T with a home-built double resonant (13)C-(1)H probehead. Three-dimensional chemical shift imaging was used to obtain (1)H-decoupled (13)C-spectra in 96-microl voxels in about 58 min. Acquisition weighting significantly reduced signal contamination and improved image quality, with no penalty in sensitivity. As a first application, infarcted hearts were studied during perfusion with [2-(13)C]-sodium acetate. The extent of the incorporation of the (13)C-label into glutamate allows us to distinguish intact and infarcted myocardium. Chemical shift images show a homogeneous glutamate distribution in intact tissue, but a negligible amount in the infarction scar.

Authors: Weidensteiner, C; Lanz, T; Horn, M; Neubauer, S; Haase, A

• Publication status: Published
• Journal: Journal of magnetic resonance (San Diego, Calif. : 1997)
• Volume: 143
• Issue: 1
• Pages: 17-23
• Publication date: 2000-03-01
• DOI: 10.1006/jmre.1999.1984
• EISSN: 1096-0856
• ISSN: 1090-7807
• Language: English
• Keywords: Rats, Wistar; Animals; Magnetic Resonance Spectroscopy; Rats; Myocardial Infarction; Carbon Isotopes; Myocardium; Glutamic Acid; Male; Image Processing, Computer-Assisted