Public health impact of isoniazid-resistant Mycobacterium tuberculosis strains with a mutation at amino-acid position 315 of katG: a decade of experience in The Netherlands.

Journal article

A previous limited study demonstrated that Mycobacterium tuberculosis isolates with a mutation at amino-acid position 315 of katG (Delta315) exhibited high-level resistance to isoniazid and were more frequently resistant to streptomycin. In the present study, isoniazid-resistant M. tuberculosis isolates from 8,332 patients in The Netherlands (1993-2002) were screened for the Delta315 mutation. Isoniazid resistance was found in 592 (7%) isolates, of which 323 (55%) carried Delta315. IS6110 restriction fragment length polymorphism analysis showed that Delta315 isolates occurred in clusters, suggesting recent transmission, at the same frequency as isoniazid-susceptible isolates. In contrast, other isoniazid-resistant isolates clustered significantly less frequently. Delta315 isolates were high-level isoniazid-resistant, streptomycin-resistant and multidrug-resistant significantly more often, and may have a greater impact on public health, than other isoniazid-resistant isolates.

Authors: Van Doorn, H; de Haas, P; Kremer, K; Vandenbroucke-Grauls, C; Borgdorff, M

• Publication status: Published
• Journal: Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
• Volume: 12
• Issue: 8
• Pages: 769-775
• Publication date: 2006-08-01
• DOI: 10.1111/j.1469-0691.2006.01495.x
• EISSN: 1469-0691
• ISSN: 1198-743X
• Language: English
• Keywords: Antitubercular Agents; Mutation; Drug Resistance, Multiple, Bacterial; Catalase; Bacterial Proteins; Adult; Middle Aged; Public Health; Mycobacterium tuberculosis; Aged; Humans; Adolescent; Isoniazid