Major histocompatibility complex (MHC) class I molecules bind peptides in the endoplasmic reticulum (ER). For this binding reaction, when performed in vitro, widely differing association rates have been reported. We have expressed empty soluble H-2Db class I molecules in Chinese hamster ovary (CHO) cells and generated complete sets of association, dissociation, and equilibrium constants of unmodified peptides using tritium-labeled peptides and stopped-flow fluorescence spectroscopy. We find t...Expand abstract
- Publication status:
- Publisher copy:
- Copyright date:
Fast association rates suggest a conformational change in the MHC class I molecule H-2Db upon peptide binding.
If you are the owner of this record, you can report an update to it here: Report update to this record