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Fast association rates suggest a conformational change in the MHC class I molecule H-2Db upon peptide binding.

Abstract:

Major histocompatibility complex (MHC) class I molecules bind peptides in the endoplasmic reticulum (ER). For this binding reaction, when performed in vitro, widely differing association rates have been reported. We have expressed empty soluble H-2Db class I molecules in Chinese hamster ovary (CHO) cells and generated complete sets of association, dissociation, and equilibrium constants of unmodified peptides using tritium-labeled peptides and stopped-flow fluorescence spectroscopy. We find t...

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Publication status:
Published

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Publisher copy:
10.1021/bi9717441

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
Journal:
Biochemistry
Volume:
37
Issue:
9
Pages:
3001-3012
Publication date:
1998-03-01
DOI:
EISSN:
1520-4995
ISSN:
0006-2960
Source identifiers:
8046
Language:
English
Keywords:
Pubs id:
pubs:8046
UUID:
uuid:5c731c67-0452-4b95-87ff-77c575d94ad5
Local pid:
pubs:8046
Deposit date:
2012-12-19

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