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Fast association rates suggest a conformational change in the MHC class I molecule H-2Db upon peptide binding.

Abstract:

Major histocompatibility complex (MHC) class I molecules bind peptides in the endoplasmic reticulum (ER). For this binding reaction, when performed in vitro, widely differing association rates have been reported. We have expressed empty soluble H-2Db class I molecules in Chinese hamster ovary (CHO) cells and generated complete sets of association, dissociation, and equilibrium constants of unmodified peptides using tritium-labeled peptides and stopped-flow fluorescence spectroscopy. We find t...

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Publication status:
Published

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Publisher copy:
10.1021/bi9717441

Authors


Springer, S More by this author
Skipper, JC More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Molecular Medicine
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Molecular Medicine, Biomedical Research Centre
Journal:
Biochemistry
Volume:
37
Issue:
9
Pages:
3001-3012
Publication date:
1998-03-05
DOI:
EISSN:
1520-4995
ISSN:
0006-2960
URN:
uuid:5c731c67-0452-4b95-87ff-77c575d94ad5
Source identifiers:
8046
Local pid:
pubs:8046

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