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Journal article : Review

Uncovering viral RNA–host cell interactions on a proteome-wide scale

Abstract:
RNA viruses interact with a wide range of cellular RNA-binding proteins (RBPs) during their life cycle. The prevalence of these host–virus interactions has been highlighted by new methods that elucidate the composition of viral ribonucleoproteins (vRNPs). Applied to 11 viruses so far, these approaches have revealed hundreds of cellular RBPs that interact with viral (v)RNA in infected cells. However, consistency across methods is limited, raising questions about methodological considerations when designing and interpreting these studies. Here, we discuss these caveats and, through comparing available vRNA interactomes, describe RBPs that are consistently identified as vRNP components and outline their potential roles in infection. In summary, these novel approaches have uncovered a new universe of host–virus interactions holding great therapeutic potential.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.tibs.2021.08.002

Authors

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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Role:
Author
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Role:
Author
ORCID:
0000-0002-4246-6082
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
ORCID:
0000-0002-7625-2648
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Sub-Department of Physical and Theoretical Chemistry
Role:
Author
ORCID:
0000-0003-2640-9560


More from this funder
Funder identifier:
https://ror.org/00k4n6c32
Funding agency for:
Kamel, W
Grant:
842067
More from this funder
Funder identifier:
https://ror.org/00cwqg982
Funding agency for:
Iselin, L
Grant:
BB/M011224/1
More from this funder
Funder identifier:
https://ror.org/03x94j517
Funding agency for:
Palmalux, N
Programme:
MRC CVR PhD programme
More from this funder
Funder identifier:
https://ror.org/03x94j517
Funding agency for:
Castello, A
Grant:
MC_UU_12014/10
MC_UU_12014/12
MR/R021562/1
MR/L019434/1


Publisher:
Cell Press
Journal:
Trends in Biochemical Sciences More from this journal
Volume:
47
Issue:
1
Pages:
23-38
Place of publication:
England
Publication date:
2021-09-09
Acceptance date:
2021-08-06
DOI:
EISSN:
1362-4326
ISSN:
0968-0004
Pmid:
34509361


Language:
English
Keywords:
Subtype:
Review
Pubs id:
1196612
Local pid:
pubs:1196612
Deposit date:
2025-02-27
ARK identifier:

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