The dependence on intracellular ATP concentration of ATP-sensitive K-channels and of Na,K-ATPase in intact HIT-T15 beta-cells.

Journal article

We have studied the effects of changes of intracellular ATP concentration ([ATP]i) on the activity of ATP-sensitive K-channels (IK(ATP] and of Na,K-ATPase in intact cells of the insulin-secreting cell-line HIT-T15. Pre-exposure of HIT beta-cells to oligomycin caused a dose-dependent reduction in [ATP]i. Marked activation of IK(ATP) activity was found when ATP was lowered below 3 mM. Na,K-ATPase was progressively inhibited as ATP was lowered to 1.5 mM. These data demonstrate that changes in in...

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Authors: Niki, I; Ashcroft, F; Ashcroft, S

• Publication status: Published
• Journal: FEBS letters
• Volume: 257
• Issue: 2
• Pages: 361-364
• Publication date: 1989-11-01
• DOI: 10.1016/0014-5793(89)81572-8
• EISSN: 1873-3468
• ISSN: 0014-5793
• Language: English
• Keywords: Deoxyglucose; Islets of Langerhans; Potassium Channels; Cytoplasm; Cell Line; Insulin; Calcium; Adenosine Triphosphate; Cell Compartmentation; Sodium-Potassium-Exchanging ATPase; Oligomycins