We have studied the effects of changes of intracellular ATP concentration ([ATP]i) on the activity of ATP-sensitive K-channels (IK(ATP] and of Na,K-ATPase in intact cells of the insulin-secreting cell-line HIT-T15. Pre-exposure of HIT beta-cells to oligomycin caused a dose-dependent reduction in [ATP]i. Marked activation of IK(ATP) activity was found when ATP was lowered below 3 mM. Na,K-ATPase was progressively inhibited as ATP was lowered to 1.5 mM. These data demonstrate that changes in in...Expand abstract
- Publication status:
- Publisher copy:
- Copyright date:
The dependence on intracellular ATP concentration of ATP-sensitive K-channels and of Na,K-ATPase in intact HIT-T15 beta-cells.
Views and Downloads
If you are the owner of this record, you can report an update to it here: Report update to this record