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Inhibition of luteinizing-hormone exocytosis by guanosine 5'-[gamma-thio]triphosphate reveals involvement of a GTP-binding protein distal to second-messenger generation.

Abstract:

Dual inhibitory and stimulatory actions of guanine nucleotides on luteinizing-hormone (LH) exocytosis were observed in primary sheep gonadotropes permeabilized with staphylococcal alpha-toxin. At resting cytosolic [Ca2+]free (pCa 7), 5'-[gamma-thio]triphosphate (GTP[S]) and guanosine 5'-[beta gamma-imido]triphosphate (p[NH]ppG) stimulated rapid LH exocytosis, which was maximal between 5 and 10 min. GTP[S] and p[NH]ppG had similar potencies (50% of maximum effect at 20-50 microM), but the effe...

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Millar, RP More by this author
Wakefield, IK More by this author
Davidson, JS More by this author
Journal:
The Biochemical journal
Volume:
275 ( Pt 2)
Pages:
399-405
Publication date:
1991-04-05
EISSN:
1470-8728
ISSN:
0264-6021
URN:
uuid:5a90cd4f-72af-45c7-b733-c568dc50d0e7
Source identifiers:
187
Local pid:
pubs:187

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