Journal article icon

Journal article

Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions.

Abstract:

Microscale thermophoresis (MST) allows for quantitative analysis of protein interactions in free solution and with low sample consumption. The technique is based on thermophoresis, the directed motion of molecules in temperature gradients. Thermophoresis is highly sensitive to all types of binding-induced changes of molecular properties, be it in size, charge, hydration shell or conformation. In an all-optical approach, an infrared laser is used for local heating, and molecule mobility in the...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1016/j.ymeth.2012.12.005

Authors


Seidel, SA More by this author
Dijkman, PM More by this author
van den Bogaart, G More by this author
Jerabek-Willemsen, M More by this author
Expand authors...
Journal:
Methods (San Diego, Calif.)
Volume:
59
Issue:
3
Pages:
301-315
Publication date:
2013-03-05
DOI:
EISSN:
1095-9130
ISSN:
1046-2023
URN:
uuid:5a5cfcc3-7a51-4756-a772-077d960bce5f
Source identifiers:
379508
Local pid:
pubs:379508

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP