Apolipoproteins L control cell death triggered by TLR3/TRIF signaling in dendritic cells.

Journal article

Apolipoproteins L (ApoLs) are Bcl-2-like proteins expressed under inflammatory conditions in myeloid and endothelial cells. We found that Toll-like receptor (TLR) stimuli, particularly the viral mimetic polyinosinic:polycytidylic acid (poly(I:C)), specifically induce ApoLs7/11 subfamilies in murine CD8α(+)  dendritic cells (DCs). This induction requires the TLR3/TRIF (where TRIF is TIR domain containing adapter-inducing interferon β) signaling pathway and is dependent on IFN-β in all ApoLs subfamilies except for ApoL7c. Poly(I:C) treatment of DCs is also associated with induction of both cell death and autophagy. ApoLs expression is related to promotion of DC death by poly(I:C), as ApoLs7/11 knockdown increases DC survival and ApoLs7 are associated with the anti-apoptotic protein Bcl-xL (where Bcl-xL is B-cell lymphoma extra large). Similarly, in human monocyte-derived DCs poly(I:C) induces both cell death and the expression of ApoLs, principally ApoL3. Finally, the BH3-like peptide of ApoLs appears to be involved in the DC death-promoting activity. We would like to propose that ApoLs are involved in cell death linked to activation of DCs by viral stimuli.

Authors: Uzureau, S; Coquerelle, C; Vermeiren, C; Uzureau, P; Van Acker, A

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Wiley
• Journal: European Journal of Immunology
• Volume: 46
• Issue: 8
• Pages: 1854-1866
• Publication date: 2016-05-01
• Acceptance date: 2016-05-12
• DOI: 10.1002/eji.201546252
• EISSN: 1521-4141
• ISSN: 0014-2980
• Pmid: 27198486
• Language: English
• Keywords: Interferon-beta; Apoptosis; Dendritic Cells; Antigens, CD8; Mice, Inbred C57BL; Cell Line; Signal Transduction; bcl-X Protein; Humans; Protein Isoforms; Toll-Like Receptor 3; Mice; Poly I-C; Adaptor Proteins, Vesicular Transport; Animals; Apolipoproteins; Cells, Cultured; Mice, Knockout