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Redox properties of flavocytochrome c3 from Shewanella frigidimarina NCIMB400.

Abstract:

The thermodynamic and catalytic properties of flavocytochrome c3 from Shewanella frigidimarina have been studied using a combination of protein film voltammetry and solution methods. As measured by solution kinetics, maximum catalytic efficiencies for fumarate reduction (kcat/Km = 2.1 x 10(7) M-1 s-1 at pH 7.2) and succinate oxidation (kcat/Km = 933 M-1 s-1 at pH 8.5) confirm that flavocytochrome c3 is a unidirectional fumarate reductase. Very similar catalytic properties are observed for the...

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Publication status:
Published

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Publisher copy:
10.1021/bi9826308

Authors


Turner, KL More by this author
Doherty, MK More by this author
Heering, HA More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Inorganic Chemistry
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Journal:
Biochemistry
Volume:
38
Issue:
11
Pages:
3302-3309
Publication date:
1999-03-05
DOI:
EISSN:
1520-4995
ISSN:
0006-2960
URN:
uuid:59be63e6-3da3-4cb6-ac07-3d1bb3468fc1
Source identifiers:
36931
Local pid:
pubs:36931

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