Journal article
Dissection of a functional interaction between the DNA translocase, FtsK, and the XerD recombinase
- Abstract:
- Successful bacterial circular chromosome segregation requires that any dimeric chromosomes, which arise by crossing over during homologous recombination, are converted to monomers. Resolution of dimers to monomers requires the action of the XerCD site-specific recombinase at dif in the chromosome replication terminus region. This reaction requires the DNA translocase, FtsKc, which activates dimer resolution by catalysing an ATP hydrolysis-dependent switch in the catalytic state of the nucleoprotein recombination complex. We show that a 62-amino-acid fragment of FtsKc interacts directly with the XerD C-terminus in order to stimulate the cleavage by XerD of BSN, a dif-DNA suicide substrate containing a nick in the 'bottom' strand. The resulting recombinase-DNA covalent complex can undergo strand exchange with intact duplex dif in the absence of ATP. Ftskc-mediated stimulation of BSN cleavage by XerD requires synaptic complex formation. Mutational impairment of the XerD-FtsKc interaction leads to reduction in the in vitro stimulation of BSN cleavage by XerD and a concomitant deficiency in the resolution of chromosomal dimers at dif in vivo, although other XerD functions are not affected.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 341.4KB, Terms of use)
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- Publisher copy:
- 10.1111/j.1365-2958.2005.05033.x
Authors
- Publisher:
- Blackwell Publishing
- Journal:
- Molecular Microbiology More from this journal
- Volume:
- 59
- Issue:
- 6
- Pages:
- 1754-1766
- Publication date:
- 2006-03-01
- Edition:
- Publisher's version
- DOI:
- EISSN:
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1365-2958
- ISSN:
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0950-382X
- Language:
-
English
- Keywords:
- Subjects:
- UUID:
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uuid:59094477-7209-4987-9f06-eedf7552fddf
- Local pid:
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ora:1692
- Deposit date:
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2008-03-14
Terms of use
- Copyright holder:
- James Yates et al
- Copyright date:
- 2006
- Notes:
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Third-parties are entitled to re-use this article, in whole or in part, in accordance with the conditions outlined in the Creative Commons Deed, Attribution 2.5 Non-Commercial, No Derivatives, which allows Open Access dissemination of your work, but does not permit commercial exploitation or the creation of derivative works. (For further details see http://creativecommons.org/licenses/by-nc-nd/2.5/).
Citation: Yates, J. et al. (2006). 'Dissection of a functional interaction between the DNA translocase, FtsK, and the XerD recombinase', Molecular Microbiology, 59 (6), 1754-1766. Available at: http://dx.doi.org/10.1111/j.1365-2958.2005.05033.x
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